The 721 spectrophotometer uses a classic optical path system and sophisticated manufacturing technology to make the instrument's test accuracy and stability much better than traditional products; it is widely used in metallurgy, chemical, machinery, medicine, biology, agriculture, environmental protection, teaching. Other industries and fields. This instrument is also an essential inspection equipment in the QS certification of food factories and drinking water plants.
Main technical indicators of 721 spectrophotometerWavelength range: 360∽800nm ​​Wavelength accuracy: 360∽600±3nm600∽700±6nm700∽800±8nm Transmittance accuracy: ±2.5% transmittance Repeatability: 0.5
Light is an electromagnetic wave with a certain wavelength and frequency. The wavelength of visible light ranges from 400 to 760 nm, the ultraviolet light is from 200 to 400 nm, and the infrared light is from 760 to 500,000 nm. Visible light presents different colors depending on the wavelength. These wavelengths display different colors of light in a certain range. The white light emitted by the sun or tungsten wire is a composite light, which is a mixed light of various monochromatic lights. The prism can be used to divide white light into various monochromatic lights arranged in wavelength order, namely red, orange, yellow, green, cyan, blue, purple, etc., which is the spectrum. The colored substance solution selectively absorbs a part of visible light energy to present different colors, and some colorless substances can characteristically select the energy of ultraviolet light or infrared light. The material absorbs certain wavelengths of light emitted by the light source to form an absorption spectrum. Because of the different molecular structure of the substance, the absorption capacity of light is different. Therefore, each substance has a specific absorption spectrum, and under certain conditions, its absorption degree is The concentration of the substance is proportional, and spectrophotometry is a method of qualitatively or quantitatively analyzing different substances by using this absorption characteristic of the substance.
In colorimetric analysis, the depth of the color of the colored substance solution is determined by the intensity of the incident light, the concentration of the colored substance solution, and the thickness of the liquid layer. When a bundle of monochromatic light illuminates the solution, the stronger the intensity of the incident light, the greater the concentration of the solution, the thicker the thickness of the liquid layer, the more the solution absorbs light, and the relationship between them conforms to the quantitative law of light absorption by the substance. That is, Lambert-Bear's law. This is the theoretical basis for spectrophotometry for quantitative analysis of materials.
Structure and function of 721 spectrophotometerThe appearance of the 721 spectrophotometer is shown in Figure 2-1. It is the most common and widely used visible spectrophotometer in domestic enterprises and institutions. It is mainly composed of light source system, spectroscopic system, measurement system and receiving display system. Four parts.
Figure 2-2 (a) Schematic diagram of the internal structure of the 721 spectrophotometer (top view)
1. Light source lamp room;
2. Power Transformers;
3. Voltage regulator circuit control board;
4. Filter electrolytic capacitor;
5. Photoelectric tube box;
6. Colorimetric part;
7. Wavelength selection friction wheel mechanism;
8. Monochromator assembly;
9. "0" coarse adjustment potentiometer;
10. Reading meter;
11. Power supply high power adjustment tube (3DD15).
Figure 2-2 (b) Schematic diagram of the internal structure of the 721 spectrophotometer (rear view)
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