The advantage of the CCK-8 test kit is that it does not require DMSO to dissolve, and is non-toxic, and operates.
Safe again
Convenient, it is very convenient and efficient for cell activity detection, cell proliferation and toxicity detection.
Details of the operation of the CCK-8 test kit in these areas, and how to choose the appropriate CCK-8 test kit?
CCK-8 Test Kit -
Cell activity assay
1. Inoculate a cell suspension (100 μl/well) in a 96-well plate. Place the plate in the incubator for a period of time (37 ° C, 5% CO)
2
).
2. Add 10 μl of CCK-8 solution to each well (be careful not to create bubbles in the wells that will affect the OD reading).
3. Incubate the plates in the incubator for 1-4 hours.
4. Determine the absorbance at 450 nm using a microplate reader.
Note: If the OD value is not measured temporarily, you can add it to each well.
10 μl 0.1 M HCL solution or 1% w/v SDS solution
And cover the culture plate in the dark to save at room temperature. The absorbance does not change within 24 hours.
CCK-8 Test Kit -
Cell proliferation-toxicity test
1. Dispense 100 μl of cell suspension (2000-5000 cells) in a 96-well plate. Place the plate in the incubator for 24 hours (37 ° C, 5% CO)
2
).
2. Add 10 μl of different concentrations of the test substance to the plate.
3. Incubate the plates in the incubator for a suitable period of time (eg, 6, 12, 24 or 48 hours).
4. Add 10 μl of CCK-8 solution to each well (do not create bubbles in the wells that will affect the OD reading).
5. Incubate the plates in the incubator for 1-4 hours.
6. Determine the absorbance at 450 nm using a microplate reader.
Note: If the OD value is not measured temporarily, you can add it to each well.
10 μl 0.1 M HCL solution or 1% w/v SDS solution
And cover the culture plate in the dark to save at room temperature. The absorbance does not change within 24 hours. If the substance to be tested is oxidizing or reducing, replace the medium with fresh medium (removal of the medium and wash the cells twice with medium, then add new medium) to remove the effects of the drug. Of course, in the case where the influence of the drug is relatively small, the medium can be directly subtracted from the blank absorption after the drug is added to the medium.
How to choose the right CCK-8 test kit?
The [CCK-8] cell proliferation/toxicity test kit currently available on the market is available in domestic or imported products. The domestically packaged CCK-8 kit has insufficient quality stability. The CCK-8 kits, which are native to domestic laboratories, have some good test results and are difficult to control in batch stability. As for the imported brands that supply CCK-8 kits, there are not many imported brands. Even Abcam, Thermo, Sigma and other brands can not provide CCK-8 kits, while other brands are far less expensive than Abbkine products.
As the first cell analysis flagship product of the Abbkine brand,
Cell Counting Kit (CCK-8)
Abbkine R&D engineers have invested a lot of money in continually exploring and optimizing the best formulas. Each effort is only to make scientific research workers more simple, efficient and stable access to valuable scientific research data.
"CCK-8 Test Kit: Comprehensive Comparison of Cell Proliferation and Toxicity Detection"
As the general agent of Abbkine China, Amy Jiejie will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates, protein purification products and Related kits, etc. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line.
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Contact Amytech Co., Ltd. for product information and complete experimental solutions.
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